Generation of the transgenic potato expressing full-length spike protein of infectious bronchitis virus.
Identifieur interne : 005345 ( Main/Exploration ); précédent : 005344; suivant : 005346Generation of the transgenic potato expressing full-length spike protein of infectious bronchitis virus.
Auteurs : Ji-Yong Zhou [République populaire de Chine] ; Li-Qin Cheng ; Xiao-Juan Zheng ; Jian-Xiang Wu ; Shao-Bin Shang ; Jin-Yong Wang ; Ji-Gang ChenSource :
- Journal of biotechnology [ 0168-1656 ] ; 2004.
Descripteurs français
- KwdFr :
- Animaux, Clonage moléculaire, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (), Glycoprotéines membranaires (biosynthèse), Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (immunologie), Ingénierie des protéines (), Poulets, Protéines de l'enveloppe virale (), Protéines de l'enveloppe virale (biosynthèse), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Protéines recombinantes (), Protéines recombinantes (biosynthèse), Protéines recombinantes (immunologie), Solanum tuberosum (génétique), Solanum tuberosum (métabolisme), Virus de la bronchite infectieuse (génétique), Virus de la bronchite infectieuse (métabolisme), Végétaux génétiquement modifiés (métabolisme).
- MESH :
- biosynthèse : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines recombinantes.
- génétique : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Solanum tuberosum, Virus de la bronchite infectieuse.
- immunologie : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines recombinantes.
- métabolisme : Solanum tuberosum, Virus de la bronchite infectieuse, Végétaux génétiquement modifiés.
- Animaux, Clonage moléculaire, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Ingénierie des protéines, Poulets, Protéines de l'enveloppe virale, Protéines recombinantes.
English descriptors
- KwdEn :
- Animals, Chickens, Cloning, Molecular, Infectious bronchitis virus (genetics), Infectious bronchitis virus (metabolism), Membrane Glycoproteins (biosynthesis), Membrane Glycoproteins (chemistry), Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Plants, Genetically Modified (metabolism), Protein Engineering (methods), Recombinant Proteins (biosynthesis), Recombinant Proteins (chemistry), Recombinant Proteins (immunology), Solanum tuberosum (genetics), Solanum tuberosum (metabolism), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (biosynthesis), Viral Envelope Proteins (chemistry), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology).
- MESH :
- chemical , biosynthesis : Membrane Glycoproteins, Recombinant Proteins, Viral Envelope Proteins.
- chemical , chemistry : Membrane Glycoproteins, Recombinant Proteins, Viral Envelope Proteins.
- genetics : Infectious bronchitis virus, Membrane Glycoproteins, Solanum tuberosum, Viral Envelope Proteins.
- chemical , immunology : Membrane Glycoproteins, Recombinant Proteins, Viral Envelope Proteins.
- metabolism : Infectious bronchitis virus, Plants, Genetically Modified, Solanum tuberosum.
- methods : Protein Engineering.
- Animals, Chickens, Cloning, Molecular, Spike Glycoprotein, Coronavirus.
Abstract
To seek a new delivery system of vaccine for infectious bronchitis virus (IBV), transgenic potato expressing full-length spike (S) protein of IBV was produced and its immunogenicity in chickens was investigated. One to three copies of S gene of IBV were randomly and stably inserted into potato (Solanum tuberrosum cv. Dongnong 303) genome by Agrobacterium tumefaciens-mediated transformation. Transcription and translation of S gene for IBV were confirmed by Northern blot and Western blot analyses in transgenic plantlets. Chickens immunized orally and intramuscularly with transgenic potato tubers expressing S protein generated the detectable levels of serum neutralizing antibodies and were protected against the challenge with the virulent IBV. In vitro secretion of interleukin 2 and T lymphocyte proliferation of spleen cells from the immunized chickens varied with the dose and the manner of vaccination with S protein derived from transgenic plants. The results indicated that S protein expressed in transgenic plants might be a new source for the production of Coronaviridae IBV vaccine.
DOI: 10.1016/j.jbiotec.2004.03.012
PubMed: 15219399
Affiliations:
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type="province">Zhejiang</region></placeName></affiliation></author><author><name sortKey="Cheng, Li Qin" sort="Cheng, Li Qin" uniqKey="Cheng L" first="Li-Qin" last="Cheng">Li-Qin Cheng</name></author><author><name sortKey="Zheng, Xiao Juan" sort="Zheng, Xiao Juan" uniqKey="Zheng X" first="Xiao-Juan" last="Zheng">Xiao-Juan Zheng</name></author><author><name sortKey="Wu, Jian Xiang" sort="Wu, Jian Xiang" uniqKey="Wu J" first="Jian-Xiang" last="Wu">Jian-Xiang Wu</name></author><author><name sortKey="Shang, Shao Bin" sort="Shang, Shao Bin" uniqKey="Shang S" first="Shao-Bin" last="Shang">Shao-Bin Shang</name></author><author><name sortKey="Wang, Jin Yong" sort="Wang, Jin Yong" uniqKey="Wang J" first="Jin-Yong" last="Wang">Jin-Yong Wang</name></author><author><name sortKey="Chen, Ji Gang" sort="Chen, Ji Gang" uniqKey="Chen J" first="Ji-Gang" last="Chen">Ji-Gang Chen</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno 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xml:lang="en">Generation of the transgenic potato expressing full-length spike protein of infectious bronchitis virus.</title><author><name sortKey="Zhou, Ji Yong" sort="Zhou, Ji Yong" uniqKey="Zhou J" first="Ji-Yong" last="Zhou">Ji-Yong Zhou</name><affiliation wicri:level="4"><nlm:affiliation>Institute of Preventive Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou 310029, China. jyzhou@zju.edu.cn</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Preventive Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou 310029</wicri:regionArea><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName><orgName type="university">Université de Zhejiang</orgName><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName></affiliation></author><author><name sortKey="Cheng, Li Qin" sort="Cheng, Li Qin" uniqKey="Cheng L" first="Li-Qin" last="Cheng">Li-Qin Cheng</name></author><author><name sortKey="Zheng, Xiao Juan" sort="Zheng, Xiao Juan" uniqKey="Zheng X" first="Xiao-Juan" last="Zheng">Xiao-Juan Zheng</name></author><author><name sortKey="Wu, Jian Xiang" sort="Wu, Jian Xiang" uniqKey="Wu J" first="Jian-Xiang" last="Wu">Jian-Xiang Wu</name></author><author><name sortKey="Shang, Shao Bin" sort="Shang, Shao Bin" uniqKey="Shang S" first="Shao-Bin" last="Shang">Shao-Bin Shang</name></author><author><name sortKey="Wang, Jin Yong" sort="Wang, Jin Yong" uniqKey="Wang J" first="Jin-Yong" last="Wang">Jin-Yong Wang</name></author><author><name sortKey="Chen, Ji Gang" sort="Chen, Ji Gang" uniqKey="Chen J" first="Ji-Gang" last="Chen">Ji-Gang Chen</name></author></analytic><series><title level="j">Journal of biotechnology</title><idno type="ISSN">0168-1656</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Chickens</term><term>Cloning, Molecular</term><term>Infectious bronchitis virus (genetics)</term><term>Infectious bronchitis virus (metabolism)</term><term>Membrane Glycoproteins (biosynthesis)</term><term>Membrane Glycoproteins (chemistry)</term><term>Membrane Glycoproteins (genetics)</term><term>Membrane Glycoproteins (immunology)</term><term>Plants, Genetically Modified (metabolism)</term><term>Protein Engineering (methods)</term><term>Recombinant Proteins (biosynthesis)</term><term>Recombinant Proteins (chemistry)</term><term>Recombinant Proteins (immunology)</term><term>Solanum tuberosum (genetics)</term><term>Solanum tuberosum (metabolism)</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins (biosynthesis)</term><term>Viral Envelope Proteins (chemistry)</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Clonage moléculaire</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires ()</term><term>Glycoprotéines membranaires (biosynthèse)</term><term>Glycoprotéines membranaires (génétique)</term><term>Glycoprotéines membranaires (immunologie)</term><term>Ingénierie des protéines ()</term><term>Poulets</term><term>Protéines de l'enveloppe virale ()</term><term>Protéines de l'enveloppe virale (biosynthèse)</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Protéines recombinantes ()</term><term>Protéines recombinantes (biosynthèse)</term><term>Protéines recombinantes (immunologie)</term><term>Solanum tuberosum (génétique)</term><term>Solanum tuberosum (métabolisme)</term><term>Virus de la bronchite infectieuse (génétique)</term><term>Virus de la bronchite infectieuse (métabolisme)</term><term>Végétaux génétiquement modifiés (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Membrane Glycoproteins</term><term>Recombinant Proteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Membrane Glycoproteins</term><term>Recombinant Proteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Infectious bronchitis virus</term><term>Membrane Glycoproteins</term><term>Solanum tuberosum</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Solanum tuberosum</term><term>Virus de la bronchite infectieuse</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Membrane Glycoproteins</term><term>Recombinant Proteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Infectious bronchitis virus</term><term>Plants, Genetically Modified</term><term>Solanum tuberosum</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Protein Engineering</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Solanum tuberosum</term><term>Virus de la bronchite infectieuse</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chickens</term><term>Cloning, Molecular</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Clonage moléculaire</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires</term><term>Ingénierie des protéines</term><term>Poulets</term><term>Protéines de l'enveloppe virale</term><term>Protéines recombinantes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To seek a new delivery system of vaccine for infectious bronchitis virus (IBV), transgenic potato expressing full-length spike (S) protein of IBV was produced and its immunogenicity in chickens was investigated. One to three copies of S gene of IBV were randomly and stably inserted into potato (Solanum tuberrosum cv. Dongnong 303) genome by Agrobacterium tumefaciens-mediated transformation. Transcription and translation of S gene for IBV were confirmed by Northern blot and Western blot analyses in transgenic plantlets. Chickens immunized orally and intramuscularly with transgenic potato tubers expressing S protein generated the detectable levels of serum neutralizing antibodies and were protected against the challenge with the virulent IBV. In vitro secretion of interleukin 2 and T lymphocyte proliferation of spleen cells from the immunized chickens varied with the dose and the manner of vaccination with S protein derived from transgenic plants. The results indicated that S protein expressed in transgenic plants might be a new source for the production of Coronaviridae IBV vaccine.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Zhejiang</li></region><settlement><li>Hangzhou</li></settlement><orgName><li>Université de Zhejiang</li></orgName></list><tree><noCountry><name sortKey="Chen, Ji Gang" sort="Chen, Ji Gang" uniqKey="Chen J" first="Ji-Gang" last="Chen">Ji-Gang Chen</name><name sortKey="Cheng, Li Qin" sort="Cheng, Li Qin" uniqKey="Cheng L" first="Li-Qin" last="Cheng">Li-Qin Cheng</name><name sortKey="Shang, Shao Bin" sort="Shang, Shao Bin" uniqKey="Shang S" first="Shao-Bin" last="Shang">Shao-Bin Shang</name><name sortKey="Wang, Jin Yong" sort="Wang, Jin Yong" uniqKey="Wang J" first="Jin-Yong" last="Wang">Jin-Yong Wang</name><name sortKey="Wu, Jian Xiang" sort="Wu, Jian Xiang" uniqKey="Wu J" first="Jian-Xiang" last="Wu">Jian-Xiang Wu</name><name sortKey="Zheng, Xiao Juan" sort="Zheng, Xiao Juan" uniqKey="Zheng X" first="Xiao-Juan" last="Zheng">Xiao-Juan Zheng</name></noCountry><country name="République populaire de Chine"><region name="Zhejiang"><name sortKey="Zhou, Ji Yong" sort="Zhou, Ji Yong" uniqKey="Zhou J" first="Ji-Yong" last="Zhou">Ji-Yong Zhou</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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